mcherry reporter Search Results


yap tead reporter  (Addgene inc)
91
Addgene inc yap tead reporter
Yap Tead Reporter, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/yap tead reporter/product/Addgene inc
Average 91 stars, based on 1 article reviews
yap tead reporter - by Bioz Stars, 2026-03
91/100 stars
  Buy from Supplier
ace reporter  (Addgene inc)
92
Addgene inc ace reporter
a <t>GFP-positive</t> <t>HEK293-ACE</t> CRISPR/Cas9 reporter cells were transfected with recombinant Cas9, gRNA, and donor template to repair DNA sequences encoding mutant non-fluorescent mCherry to functional fluorescent mCherry expression cassettes. b The percentages of HEK293-ACE CRISPR/Cas9 reporter cells mock stimulated and stimulated with cGAMP determined to be mCherry positive are presented ( n = cell culture replicates, data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). c The percentages of HEK293-ACE cells stably expressing cGAS and control (empty plasmid) determined by flow cytometry to be mCherry positive are presented (data presented are mean ± s.d., n = 4 cell culture replicates, data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). d GFP-positive HEK293-ACE CRISPR/Cas9 reporter cells were treated with human recombinant interferon-β (50 ng/ml) and then transfected with recombinant Cas9, gRNA, and donor template to repair DNA sequences encoding mutant non-fluorescent mCherry to functional fluorescent mCherry expression cassettes, followed by flow cytometry. Quantification of flow cytometry data is presented ( n = 5 cell culture replicate; data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). e The Rosa26 locus was edited using CRISPR/Cas9 in WT, cGAS −/− , cGAS (GS198AA) , Sting −/− , and Ifnar −/− mouse primary embryonic fibroblasts and subsequently PCR amplified for next-generation sequencing and CRISPResso analysis. The frequency of CRISPR/Cas9-mediated homology-directed repair outcomes in these genotypes are presented ( N = 19 for WT, N = 19 for cGAS −/− , N = 17 for cGAS (GS198AA) , N = 19 for Sting −/− , and N = 19 for Ifnar −/− cell culture replicates; data presented are mean ± s.d.; two-tailed unpaired t test, no adjustments were made for multiple comparisons; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). Each data point represents percentage of sequence reads, indicative of HDR, n = 19 cell culture replicates for each genotype. f The frequency of CRISPR/Cas9-mediated genome-editing outcomes in mouse embryos in the presence or absence of cGAMP was determined as described in the schematic (Supplementary Fig. ; n = 26 embryos for vehicle, n = 18 embryos for cGAMP; data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). Each data point represents percentage of sequence reads within the indicated outcomes (Total edits, NHEJ, HDR).
Ace Reporter, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ace reporter/product/Addgene inc
Average 92 stars, based on 1 article reviews
ace reporter - by Bioz Stars, 2026-03
92/100 stars
  Buy from Supplier
nes mcherry nls reporter  (Addgene inc)
94
Addgene inc nes mcherry nls reporter
a <t>GFP-positive</t> <t>HEK293-ACE</t> CRISPR/Cas9 reporter cells were transfected with recombinant Cas9, gRNA, and donor template to repair DNA sequences encoding mutant non-fluorescent mCherry to functional fluorescent mCherry expression cassettes. b The percentages of HEK293-ACE CRISPR/Cas9 reporter cells mock stimulated and stimulated with cGAMP determined to be mCherry positive are presented ( n = cell culture replicates, data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). c The percentages of HEK293-ACE cells stably expressing cGAS and control (empty plasmid) determined by flow cytometry to be mCherry positive are presented (data presented are mean ± s.d., n = 4 cell culture replicates, data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). d GFP-positive HEK293-ACE CRISPR/Cas9 reporter cells were treated with human recombinant interferon-β (50 ng/ml) and then transfected with recombinant Cas9, gRNA, and donor template to repair DNA sequences encoding mutant non-fluorescent mCherry to functional fluorescent mCherry expression cassettes, followed by flow cytometry. Quantification of flow cytometry data is presented ( n = 5 cell culture replicate; data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). e The Rosa26 locus was edited using CRISPR/Cas9 in WT, cGAS −/− , cGAS (GS198AA) , Sting −/− , and Ifnar −/− mouse primary embryonic fibroblasts and subsequently PCR amplified for next-generation sequencing and CRISPResso analysis. The frequency of CRISPR/Cas9-mediated homology-directed repair outcomes in these genotypes are presented ( N = 19 for WT, N = 19 for cGAS −/− , N = 17 for cGAS (GS198AA) , N = 19 for Sting −/− , and N = 19 for Ifnar −/− cell culture replicates; data presented are mean ± s.d.; two-tailed unpaired t test, no adjustments were made for multiple comparisons; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). Each data point represents percentage of sequence reads, indicative of HDR, n = 19 cell culture replicates for each genotype. f The frequency of CRISPR/Cas9-mediated genome-editing outcomes in mouse embryos in the presence or absence of cGAMP was determined as described in the schematic (Supplementary Fig. ; n = 26 embryos for vehicle, n = 18 embryos for cGAMP; data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). Each data point represents percentage of sequence reads within the indicated outcomes (Total edits, NHEJ, HDR).
Nes Mcherry Nls Reporter, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nes mcherry nls reporter/product/Addgene inc
Average 94 stars, based on 1 article reviews
nes mcherry nls reporter - by Bioz Stars, 2026-03
94/100 stars
  Buy from Supplier
tet inducible mcherry reporter  (Addgene inc)
92
Addgene inc tet inducible mcherry reporter
a <t>GFP-positive</t> <t>HEK293-ACE</t> CRISPR/Cas9 reporter cells were transfected with recombinant Cas9, gRNA, and donor template to repair DNA sequences encoding mutant non-fluorescent mCherry to functional fluorescent mCherry expression cassettes. b The percentages of HEK293-ACE CRISPR/Cas9 reporter cells mock stimulated and stimulated with cGAMP determined to be mCherry positive are presented ( n = cell culture replicates, data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). c The percentages of HEK293-ACE cells stably expressing cGAS and control (empty plasmid) determined by flow cytometry to be mCherry positive are presented (data presented are mean ± s.d., n = 4 cell culture replicates, data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). d GFP-positive HEK293-ACE CRISPR/Cas9 reporter cells were treated with human recombinant interferon-β (50 ng/ml) and then transfected with recombinant Cas9, gRNA, and donor template to repair DNA sequences encoding mutant non-fluorescent mCherry to functional fluorescent mCherry expression cassettes, followed by flow cytometry. Quantification of flow cytometry data is presented ( n = 5 cell culture replicate; data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). e The Rosa26 locus was edited using CRISPR/Cas9 in WT, cGAS −/− , cGAS (GS198AA) , Sting −/− , and Ifnar −/− mouse primary embryonic fibroblasts and subsequently PCR amplified for next-generation sequencing and CRISPResso analysis. The frequency of CRISPR/Cas9-mediated homology-directed repair outcomes in these genotypes are presented ( N = 19 for WT, N = 19 for cGAS −/− , N = 17 for cGAS (GS198AA) , N = 19 for Sting −/− , and N = 19 for Ifnar −/− cell culture replicates; data presented are mean ± s.d.; two-tailed unpaired t test, no adjustments were made for multiple comparisons; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). Each data point represents percentage of sequence reads, indicative of HDR, n = 19 cell culture replicates for each genotype. f The frequency of CRISPR/Cas9-mediated genome-editing outcomes in mouse embryos in the presence or absence of cGAMP was determined as described in the schematic (Supplementary Fig. ; n = 26 embryos for vehicle, n = 18 embryos for cGAMP; data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). Each data point represents percentage of sequence reads within the indicated outcomes (Total edits, NHEJ, HDR).
Tet Inducible Mcherry Reporter, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tet inducible mcherry reporter/product/Addgene inc
Average 92 stars, based on 1 article reviews
tet inducible mcherry reporter - by Bioz Stars, 2026-03
92/100 stars
  Buy from Supplier
mcherry reporter  (Addgene inc)
94
Addgene inc mcherry reporter
a <t>GFP-positive</t> <t>HEK293-ACE</t> CRISPR/Cas9 reporter cells were transfected with recombinant Cas9, gRNA, and donor template to repair DNA sequences encoding mutant non-fluorescent mCherry to functional fluorescent mCherry expression cassettes. b The percentages of HEK293-ACE CRISPR/Cas9 reporter cells mock stimulated and stimulated with cGAMP determined to be mCherry positive are presented ( n = cell culture replicates, data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). c The percentages of HEK293-ACE cells stably expressing cGAS and control (empty plasmid) determined by flow cytometry to be mCherry positive are presented (data presented are mean ± s.d., n = 4 cell culture replicates, data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). d GFP-positive HEK293-ACE CRISPR/Cas9 reporter cells were treated with human recombinant interferon-β (50 ng/ml) and then transfected with recombinant Cas9, gRNA, and donor template to repair DNA sequences encoding mutant non-fluorescent mCherry to functional fluorescent mCherry expression cassettes, followed by flow cytometry. Quantification of flow cytometry data is presented ( n = 5 cell culture replicate; data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). e The Rosa26 locus was edited using CRISPR/Cas9 in WT, cGAS −/− , cGAS (GS198AA) , Sting −/− , and Ifnar −/− mouse primary embryonic fibroblasts and subsequently PCR amplified for next-generation sequencing and CRISPResso analysis. The frequency of CRISPR/Cas9-mediated homology-directed repair outcomes in these genotypes are presented ( N = 19 for WT, N = 19 for cGAS −/− , N = 17 for cGAS (GS198AA) , N = 19 for Sting −/− , and N = 19 for Ifnar −/− cell culture replicates; data presented are mean ± s.d.; two-tailed unpaired t test, no adjustments were made for multiple comparisons; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). Each data point represents percentage of sequence reads, indicative of HDR, n = 19 cell culture replicates for each genotype. f The frequency of CRISPR/Cas9-mediated genome-editing outcomes in mouse embryos in the presence or absence of cGAMP was determined as described in the schematic (Supplementary Fig. ; n = 26 embryos for vehicle, n = 18 embryos for cGAMP; data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). Each data point represents percentage of sequence reads within the indicated outcomes (Total edits, NHEJ, HDR).
Mcherry Reporter, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mcherry reporter/product/Addgene inc
Average 94 stars, based on 1 article reviews
mcherry reporter - by Bioz Stars, 2026-03
94/100 stars
  Buy from Supplier
isre-mcherry reporter plasmid  (Cellecta Inc)
90
Cellecta Inc isre-mcherry reporter plasmid
a <t>GFP-positive</t> <t>HEK293-ACE</t> CRISPR/Cas9 reporter cells were transfected with recombinant Cas9, gRNA, and donor template to repair DNA sequences encoding mutant non-fluorescent mCherry to functional fluorescent mCherry expression cassettes. b The percentages of HEK293-ACE CRISPR/Cas9 reporter cells mock stimulated and stimulated with cGAMP determined to be mCherry positive are presented ( n = cell culture replicates, data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). c The percentages of HEK293-ACE cells stably expressing cGAS and control (empty plasmid) determined by flow cytometry to be mCherry positive are presented (data presented are mean ± s.d., n = 4 cell culture replicates, data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). d GFP-positive HEK293-ACE CRISPR/Cas9 reporter cells were treated with human recombinant interferon-β (50 ng/ml) and then transfected with recombinant Cas9, gRNA, and donor template to repair DNA sequences encoding mutant non-fluorescent mCherry to functional fluorescent mCherry expression cassettes, followed by flow cytometry. Quantification of flow cytometry data is presented ( n = 5 cell culture replicate; data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). e The Rosa26 locus was edited using CRISPR/Cas9 in WT, cGAS −/− , cGAS (GS198AA) , Sting −/− , and Ifnar −/− mouse primary embryonic fibroblasts and subsequently PCR amplified for next-generation sequencing and CRISPResso analysis. The frequency of CRISPR/Cas9-mediated homology-directed repair outcomes in these genotypes are presented ( N = 19 for WT, N = 19 for cGAS −/− , N = 17 for cGAS (GS198AA) , N = 19 for Sting −/− , and N = 19 for Ifnar −/− cell culture replicates; data presented are mean ± s.d.; two-tailed unpaired t test, no adjustments were made for multiple comparisons; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). Each data point represents percentage of sequence reads, indicative of HDR, n = 19 cell culture replicates for each genotype. f The frequency of CRISPR/Cas9-mediated genome-editing outcomes in mouse embryos in the presence or absence of cGAMP was determined as described in the schematic (Supplementary Fig. ; n = 26 embryos for vehicle, n = 18 embryos for cGAMP; data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). Each data point represents percentage of sequence reads within the indicated outcomes (Total edits, NHEJ, HDR).
Isre Mcherry Reporter Plasmid, supplied by Cellecta Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/isre-mcherry reporter plasmid/product/Cellecta Inc
Average 90 stars, based on 1 article reviews
isre-mcherry reporter plasmid - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
recombinant adenovirus ad-nfat5  (Cyagen Biosciences)
90
Cyagen Biosciences recombinant adenovirus ad-nfat5
a <t>GFP-positive</t> <t>HEK293-ACE</t> CRISPR/Cas9 reporter cells were transfected with recombinant Cas9, gRNA, and donor template to repair DNA sequences encoding mutant non-fluorescent mCherry to functional fluorescent mCherry expression cassettes. b The percentages of HEK293-ACE CRISPR/Cas9 reporter cells mock stimulated and stimulated with cGAMP determined to be mCherry positive are presented ( n = cell culture replicates, data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). c The percentages of HEK293-ACE cells stably expressing cGAS and control (empty plasmid) determined by flow cytometry to be mCherry positive are presented (data presented are mean ± s.d., n = 4 cell culture replicates, data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). d GFP-positive HEK293-ACE CRISPR/Cas9 reporter cells were treated with human recombinant interferon-β (50 ng/ml) and then transfected with recombinant Cas9, gRNA, and donor template to repair DNA sequences encoding mutant non-fluorescent mCherry to functional fluorescent mCherry expression cassettes, followed by flow cytometry. Quantification of flow cytometry data is presented ( n = 5 cell culture replicate; data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). e The Rosa26 locus was edited using CRISPR/Cas9 in WT, cGAS −/− , cGAS (GS198AA) , Sting −/− , and Ifnar −/− mouse primary embryonic fibroblasts and subsequently PCR amplified for next-generation sequencing and CRISPResso analysis. The frequency of CRISPR/Cas9-mediated homology-directed repair outcomes in these genotypes are presented ( N = 19 for WT, N = 19 for cGAS −/− , N = 17 for cGAS (GS198AA) , N = 19 for Sting −/− , and N = 19 for Ifnar −/− cell culture replicates; data presented are mean ± s.d.; two-tailed unpaired t test, no adjustments were made for multiple comparisons; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). Each data point represents percentage of sequence reads, indicative of HDR, n = 19 cell culture replicates for each genotype. f The frequency of CRISPR/Cas9-mediated genome-editing outcomes in mouse embryos in the presence or absence of cGAMP was determined as described in the schematic (Supplementary Fig. ; n = 26 embryos for vehicle, n = 18 embryos for cGAMP; data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). Each data point represents percentage of sequence reads within the indicated outcomes (Total edits, NHEJ, HDR).
Recombinant Adenovirus Ad Nfat5, supplied by Cyagen Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant adenovirus ad-nfat5/product/Cyagen Biosciences
Average 90 stars, based on 1 article reviews
recombinant adenovirus ad-nfat5 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
renal tubular cell-specific mcherry-green fluorescent protein (gfp)-lc3 autophagy reporter mice  (Shanghai Model Organisms Center)
90
Shanghai Model Organisms Center renal tubular cell-specific mcherry-green fluorescent protein (gfp)-lc3 autophagy reporter mice
a <t>GFP-positive</t> <t>HEK293-ACE</t> CRISPR/Cas9 reporter cells were transfected with recombinant Cas9, gRNA, and donor template to repair DNA sequences encoding mutant non-fluorescent mCherry to functional fluorescent mCherry expression cassettes. b The percentages of HEK293-ACE CRISPR/Cas9 reporter cells mock stimulated and stimulated with cGAMP determined to be mCherry positive are presented ( n = cell culture replicates, data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). c The percentages of HEK293-ACE cells stably expressing cGAS and control (empty plasmid) determined by flow cytometry to be mCherry positive are presented (data presented are mean ± s.d., n = 4 cell culture replicates, data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). d GFP-positive HEK293-ACE CRISPR/Cas9 reporter cells were treated with human recombinant interferon-β (50 ng/ml) and then transfected with recombinant Cas9, gRNA, and donor template to repair DNA sequences encoding mutant non-fluorescent mCherry to functional fluorescent mCherry expression cassettes, followed by flow cytometry. Quantification of flow cytometry data is presented ( n = 5 cell culture replicate; data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). e The Rosa26 locus was edited using CRISPR/Cas9 in WT, cGAS −/− , cGAS (GS198AA) , Sting −/− , and Ifnar −/− mouse primary embryonic fibroblasts and subsequently PCR amplified for next-generation sequencing and CRISPResso analysis. The frequency of CRISPR/Cas9-mediated homology-directed repair outcomes in these genotypes are presented ( N = 19 for WT, N = 19 for cGAS −/− , N = 17 for cGAS (GS198AA) , N = 19 for Sting −/− , and N = 19 for Ifnar −/− cell culture replicates; data presented are mean ± s.d.; two-tailed unpaired t test, no adjustments were made for multiple comparisons; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). Each data point represents percentage of sequence reads, indicative of HDR, n = 19 cell culture replicates for each genotype. f The frequency of CRISPR/Cas9-mediated genome-editing outcomes in mouse embryos in the presence or absence of cGAMP was determined as described in the schematic (Supplementary Fig. ; n = 26 embryos for vehicle, n = 18 embryos for cGAMP; data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). Each data point represents percentage of sequence reads within the indicated outcomes (Total edits, NHEJ, HDR).
Renal Tubular Cell Specific Mcherry Green Fluorescent Protein (Gfp) Lc3 Autophagy Reporter Mice, supplied by Shanghai Model Organisms Center, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/renal tubular cell-specific mcherry-green fluorescent protein (gfp)-lc3 autophagy reporter mice/product/Shanghai Model Organisms Center
Average 90 stars, based on 1 article reviews
renal tubular cell-specific mcherry-green fluorescent protein (gfp)-lc3 autophagy reporter mice - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
lentiviruses encoding for shrnas for usp46 silencing with the mcherry reporter  (VectorBuilder GmbH)
90
VectorBuilder GmbH lentiviruses encoding for shrnas for usp46 silencing with the mcherry reporter
a <t>GFP-positive</t> <t>HEK293-ACE</t> CRISPR/Cas9 reporter cells were transfected with recombinant Cas9, gRNA, and donor template to repair DNA sequences encoding mutant non-fluorescent mCherry to functional fluorescent mCherry expression cassettes. b The percentages of HEK293-ACE CRISPR/Cas9 reporter cells mock stimulated and stimulated with cGAMP determined to be mCherry positive are presented ( n = cell culture replicates, data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). c The percentages of HEK293-ACE cells stably expressing cGAS and control (empty plasmid) determined by flow cytometry to be mCherry positive are presented (data presented are mean ± s.d., n = 4 cell culture replicates, data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). d GFP-positive HEK293-ACE CRISPR/Cas9 reporter cells were treated with human recombinant interferon-β (50 ng/ml) and then transfected with recombinant Cas9, gRNA, and donor template to repair DNA sequences encoding mutant non-fluorescent mCherry to functional fluorescent mCherry expression cassettes, followed by flow cytometry. Quantification of flow cytometry data is presented ( n = 5 cell culture replicate; data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). e The Rosa26 locus was edited using CRISPR/Cas9 in WT, cGAS −/− , cGAS (GS198AA) , Sting −/− , and Ifnar −/− mouse primary embryonic fibroblasts and subsequently PCR amplified for next-generation sequencing and CRISPResso analysis. The frequency of CRISPR/Cas9-mediated homology-directed repair outcomes in these genotypes are presented ( N = 19 for WT, N = 19 for cGAS −/− , N = 17 for cGAS (GS198AA) , N = 19 for Sting −/− , and N = 19 for Ifnar −/− cell culture replicates; data presented are mean ± s.d.; two-tailed unpaired t test, no adjustments were made for multiple comparisons; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). Each data point represents percentage of sequence reads, indicative of HDR, n = 19 cell culture replicates for each genotype. f The frequency of CRISPR/Cas9-mediated genome-editing outcomes in mouse embryos in the presence or absence of cGAMP was determined as described in the schematic (Supplementary Fig. ; n = 26 embryos for vehicle, n = 18 embryos for cGAMP; data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). Each data point represents percentage of sequence reads within the indicated outcomes (Total edits, NHEJ, HDR).
Lentiviruses Encoding For Shrnas For Usp46 Silencing With The Mcherry Reporter, supplied by VectorBuilder GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lentiviruses encoding for shrnas for usp46 silencing with the mcherry reporter/product/VectorBuilder GmbH
Average 90 stars, based on 1 article reviews
lentiviruses encoding for shrnas for usp46 silencing with the mcherry reporter - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
shrna and mcherry reporters  (System Biosciences Inc)
90
System Biosciences Inc shrna and mcherry reporters
a <t>GFP-positive</t> <t>HEK293-ACE</t> CRISPR/Cas9 reporter cells were transfected with recombinant Cas9, gRNA, and donor template to repair DNA sequences encoding mutant non-fluorescent mCherry to functional fluorescent mCherry expression cassettes. b The percentages of HEK293-ACE CRISPR/Cas9 reporter cells mock stimulated and stimulated with cGAMP determined to be mCherry positive are presented ( n = cell culture replicates, data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). c The percentages of HEK293-ACE cells stably expressing cGAS and control (empty plasmid) determined by flow cytometry to be mCherry positive are presented (data presented are mean ± s.d., n = 4 cell culture replicates, data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). d GFP-positive HEK293-ACE CRISPR/Cas9 reporter cells were treated with human recombinant interferon-β (50 ng/ml) and then transfected with recombinant Cas9, gRNA, and donor template to repair DNA sequences encoding mutant non-fluorescent mCherry to functional fluorescent mCherry expression cassettes, followed by flow cytometry. Quantification of flow cytometry data is presented ( n = 5 cell culture replicate; data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). e The Rosa26 locus was edited using CRISPR/Cas9 in WT, cGAS −/− , cGAS (GS198AA) , Sting −/− , and Ifnar −/− mouse primary embryonic fibroblasts and subsequently PCR amplified for next-generation sequencing and CRISPResso analysis. The frequency of CRISPR/Cas9-mediated homology-directed repair outcomes in these genotypes are presented ( N = 19 for WT, N = 19 for cGAS −/− , N = 17 for cGAS (GS198AA) , N = 19 for Sting −/− , and N = 19 for Ifnar −/− cell culture replicates; data presented are mean ± s.d.; two-tailed unpaired t test, no adjustments were made for multiple comparisons; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). Each data point represents percentage of sequence reads, indicative of HDR, n = 19 cell culture replicates for each genotype. f The frequency of CRISPR/Cas9-mediated genome-editing outcomes in mouse embryos in the presence or absence of cGAMP was determined as described in the schematic (Supplementary Fig. ; n = 26 embryos for vehicle, n = 18 embryos for cGAMP; data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). Each data point represents percentage of sequence reads within the indicated outcomes (Total edits, NHEJ, HDR).
Shrna And Mcherry Reporters, supplied by System Biosciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/shrna and mcherry reporters/product/System Biosciences Inc
Average 90 stars, based on 1 article reviews
shrna and mcherry reporters - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
reporter dna plasmid ctm-mcherry  (Yeasen Biotechnology)
90
Yeasen Biotechnology reporter dna plasmid ctm-mcherry
a <t>GFP-positive</t> <t>HEK293-ACE</t> CRISPR/Cas9 reporter cells were transfected with recombinant Cas9, gRNA, and donor template to repair DNA sequences encoding mutant non-fluorescent mCherry to functional fluorescent mCherry expression cassettes. b The percentages of HEK293-ACE CRISPR/Cas9 reporter cells mock stimulated and stimulated with cGAMP determined to be mCherry positive are presented ( n = cell culture replicates, data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). c The percentages of HEK293-ACE cells stably expressing cGAS and control (empty plasmid) determined by flow cytometry to be mCherry positive are presented (data presented are mean ± s.d., n = 4 cell culture replicates, data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). d GFP-positive HEK293-ACE CRISPR/Cas9 reporter cells were treated with human recombinant interferon-β (50 ng/ml) and then transfected with recombinant Cas9, gRNA, and donor template to repair DNA sequences encoding mutant non-fluorescent mCherry to functional fluorescent mCherry expression cassettes, followed by flow cytometry. Quantification of flow cytometry data is presented ( n = 5 cell culture replicate; data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). e The Rosa26 locus was edited using CRISPR/Cas9 in WT, cGAS −/− , cGAS (GS198AA) , Sting −/− , and Ifnar −/− mouse primary embryonic fibroblasts and subsequently PCR amplified for next-generation sequencing and CRISPResso analysis. The frequency of CRISPR/Cas9-mediated homology-directed repair outcomes in these genotypes are presented ( N = 19 for WT, N = 19 for cGAS −/− , N = 17 for cGAS (GS198AA) , N = 19 for Sting −/− , and N = 19 for Ifnar −/− cell culture replicates; data presented are mean ± s.d.; two-tailed unpaired t test, no adjustments were made for multiple comparisons; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). Each data point represents percentage of sequence reads, indicative of HDR, n = 19 cell culture replicates for each genotype. f The frequency of CRISPR/Cas9-mediated genome-editing outcomes in mouse embryos in the presence or absence of cGAMP was determined as described in the schematic (Supplementary Fig. ; n = 26 embryos for vehicle, n = 18 embryos for cGAMP; data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). Each data point represents percentage of sequence reads within the indicated outcomes (Total edits, NHEJ, HDR).
Reporter Dna Plasmid Ctm Mcherry, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/reporter dna plasmid ctm-mcherry/product/Yeasen Biotechnology
Average 90 stars, based on 1 article reviews
reporter dna plasmid ctm-mcherry - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
mcherry-gfp-map1lc3b (from rat) from the rosa26 locus  (TaconicArtemis gmbh)
90
TaconicArtemis gmbh mcherry-gfp-map1lc3b (from rat) from the rosa26 locus
a <t>GFP-positive</t> <t>HEK293-ACE</t> CRISPR/Cas9 reporter cells were transfected with recombinant Cas9, gRNA, and donor template to repair DNA sequences encoding mutant non-fluorescent mCherry to functional fluorescent mCherry expression cassettes. b The percentages of HEK293-ACE CRISPR/Cas9 reporter cells mock stimulated and stimulated with cGAMP determined to be mCherry positive are presented ( n = cell culture replicates, data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). c The percentages of HEK293-ACE cells stably expressing cGAS and control (empty plasmid) determined by flow cytometry to be mCherry positive are presented (data presented are mean ± s.d., n = 4 cell culture replicates, data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). d GFP-positive HEK293-ACE CRISPR/Cas9 reporter cells were treated with human recombinant interferon-β (50 ng/ml) and then transfected with recombinant Cas9, gRNA, and donor template to repair DNA sequences encoding mutant non-fluorescent mCherry to functional fluorescent mCherry expression cassettes, followed by flow cytometry. Quantification of flow cytometry data is presented ( n = 5 cell culture replicate; data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). e The Rosa26 locus was edited using CRISPR/Cas9 in WT, cGAS −/− , cGAS (GS198AA) , Sting −/− , and Ifnar −/− mouse primary embryonic fibroblasts and subsequently PCR amplified for next-generation sequencing and CRISPResso analysis. The frequency of CRISPR/Cas9-mediated homology-directed repair outcomes in these genotypes are presented ( N = 19 for WT, N = 19 for cGAS −/− , N = 17 for cGAS (GS198AA) , N = 19 for Sting −/− , and N = 19 for Ifnar −/− cell culture replicates; data presented are mean ± s.d.; two-tailed unpaired t test, no adjustments were made for multiple comparisons; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). Each data point represents percentage of sequence reads, indicative of HDR, n = 19 cell culture replicates for each genotype. f The frequency of CRISPR/Cas9-mediated genome-editing outcomes in mouse embryos in the presence or absence of cGAMP was determined as described in the schematic (Supplementary Fig. ; n = 26 embryos for vehicle, n = 18 embryos for cGAMP; data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). Each data point represents percentage of sequence reads within the indicated outcomes (Total edits, NHEJ, HDR).
Mcherry Gfp Map1lc3b (From Rat) From The Rosa26 Locus, supplied by TaconicArtemis gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mcherry-gfp-map1lc3b (from rat) from the rosa26 locus/product/TaconicArtemis gmbh
Average 90 stars, based on 1 article reviews
mcherry-gfp-map1lc3b (from rat) from the rosa26 locus - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


a GFP-positive HEK293-ACE CRISPR/Cas9 reporter cells were transfected with recombinant Cas9, gRNA, and donor template to repair DNA sequences encoding mutant non-fluorescent mCherry to functional fluorescent mCherry expression cassettes. b The percentages of HEK293-ACE CRISPR/Cas9 reporter cells mock stimulated and stimulated with cGAMP determined to be mCherry positive are presented ( n = cell culture replicates, data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). c The percentages of HEK293-ACE cells stably expressing cGAS and control (empty plasmid) determined by flow cytometry to be mCherry positive are presented (data presented are mean ± s.d., n = 4 cell culture replicates, data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). d GFP-positive HEK293-ACE CRISPR/Cas9 reporter cells were treated with human recombinant interferon-β (50 ng/ml) and then transfected with recombinant Cas9, gRNA, and donor template to repair DNA sequences encoding mutant non-fluorescent mCherry to functional fluorescent mCherry expression cassettes, followed by flow cytometry. Quantification of flow cytometry data is presented ( n = 5 cell culture replicate; data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). e The Rosa26 locus was edited using CRISPR/Cas9 in WT, cGAS −/− , cGAS (GS198AA) , Sting −/− , and Ifnar −/− mouse primary embryonic fibroblasts and subsequently PCR amplified for next-generation sequencing and CRISPResso analysis. The frequency of CRISPR/Cas9-mediated homology-directed repair outcomes in these genotypes are presented ( N = 19 for WT, N = 19 for cGAS −/− , N = 17 for cGAS (GS198AA) , N = 19 for Sting −/− , and N = 19 for Ifnar −/− cell culture replicates; data presented are mean ± s.d.; two-tailed unpaired t test, no adjustments were made for multiple comparisons; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). Each data point represents percentage of sequence reads, indicative of HDR, n = 19 cell culture replicates for each genotype. f The frequency of CRISPR/Cas9-mediated genome-editing outcomes in mouse embryos in the presence or absence of cGAMP was determined as described in the schematic (Supplementary Fig. ; n = 26 embryos for vehicle, n = 18 embryos for cGAMP; data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). Each data point represents percentage of sequence reads within the indicated outcomes (Total edits, NHEJ, HDR).

Journal: Nature Communications

Article Title: A non-canonical, interferon-independent signaling activity of cGAMP triggers DNA damage response signaling

doi: 10.1038/s41467-021-26240-9

Figure Lengend Snippet: a GFP-positive HEK293-ACE CRISPR/Cas9 reporter cells were transfected with recombinant Cas9, gRNA, and donor template to repair DNA sequences encoding mutant non-fluorescent mCherry to functional fluorescent mCherry expression cassettes. b The percentages of HEK293-ACE CRISPR/Cas9 reporter cells mock stimulated and stimulated with cGAMP determined to be mCherry positive are presented ( n = cell culture replicates, data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). c The percentages of HEK293-ACE cells stably expressing cGAS and control (empty plasmid) determined by flow cytometry to be mCherry positive are presented (data presented are mean ± s.d., n = 4 cell culture replicates, data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). d GFP-positive HEK293-ACE CRISPR/Cas9 reporter cells were treated with human recombinant interferon-β (50 ng/ml) and then transfected with recombinant Cas9, gRNA, and donor template to repair DNA sequences encoding mutant non-fluorescent mCherry to functional fluorescent mCherry expression cassettes, followed by flow cytometry. Quantification of flow cytometry data is presented ( n = 5 cell culture replicate; data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). e The Rosa26 locus was edited using CRISPR/Cas9 in WT, cGAS −/− , cGAS (GS198AA) , Sting −/− , and Ifnar −/− mouse primary embryonic fibroblasts and subsequently PCR amplified for next-generation sequencing and CRISPResso analysis. The frequency of CRISPR/Cas9-mediated homology-directed repair outcomes in these genotypes are presented ( N = 19 for WT, N = 19 for cGAS −/− , N = 17 for cGAS (GS198AA) , N = 19 for Sting −/− , and N = 19 for Ifnar −/− cell culture replicates; data presented are mean ± s.d.; two-tailed unpaired t test, no adjustments were made for multiple comparisons; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). Each data point represents percentage of sequence reads, indicative of HDR, n = 19 cell culture replicates for each genotype. f The frequency of CRISPR/Cas9-mediated genome-editing outcomes in mouse embryos in the presence or absence of cGAMP was determined as described in the schematic (Supplementary Fig. ; n = 26 embryos for vehicle, n = 18 embryos for cGAMP; data presented are mean ± s.d.; two-tailed unpaired t test; * p < 0.05 indicates significance compared to respective groups; ns indicates not significant). Each data point represents percentage of sequence reads within the indicated outcomes (Total edits, NHEJ, HDR).

Article Snippet: The ACE reporter (Addgene #109428) described previously , was integrated in HEK293 cells using lentiviral transduction.

Techniques: CRISPR, Transfection, Recombinant, Mutagenesis, Functional Assay, Expressing, Cell Culture, Two Tailed Test, Stable Transfection, Control, Plasmid Preparation, Flow Cytometry, Amplification, Next-Generation Sequencing, Sequencing